Biocatalysis and integration of metabolism 357

Module code BCM 357
Qualification Undergraduate
Faculty Faculty of Natural and Agricultural Sciences
Module content

Enzyme nomenclature and classification. Specificity and mechanisms: the active site, mechanisms of catalysis and examples of specific enzyme mechanisms, e.g. lysozyme and carboxypeptidase A. Advanced enzyme kinetics, Cleland nomenclature and multi-substrate reactions. Allosteric enzymes: models by Koshland, Hill and Monod. Ligands binding to proteins. Inhibitors of angiotensin converting enzyme (ACE). RNA as enzymes. Applications of enzymes in food and cosmetics industries and in clinical pathology assays as biomarkers of diseases and toxic responses. Elucidation of metabolic pathways. Antibiotic resistance mechanisms and development of new drugs. Hormonal regulation and integration of mammalian metabolism. Regulation of fuel metabolism after a meal, period between meals and starvation. Metabolic adaptions during diabetes. Obesity and the regulation of body mass. Obesity, matabolic syndrom and Type 2 diabetes (T2D). Management of T2D with diet, exercise and medication. Practical sessions cover tutorials on calculations, isolation of an enzyme, determination of pH and temperature optimum, determination of Km and Vmax, enzyme activation and enzyme inhibition. Computer simulations of drug design and protein docking to improve efficacy and lower toxicity.

Module credits 18.00
Prerequisites BCM 251 and BCM 252 and BCM 261 or permission from the HOD
Contact time 2 lectures per week, 1 practical/tutorial per week
Language of tuition Module is presented in English
Department Biochemistry, Genetics and Microbiology
Period of presentation Semester 1

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