South Africa hosts two of the world’s largest individually timed one day cycle races with approximately 35 000 cyclists per race. Despite the fact that these events are major international drawcards and that cycling is a popular recreational activity globally, there is limited research on the use of nutrition and supplements in amateur cyclists. Most research has been done in Europe and America. Much of the current body of knowledge has been gleaned from cyclists involved in cycle tours typically 5 days or longer or from professional cyclists attending training camps. The purpose of this study was to analyse the nutrition and supplement practices of amateur cyclists involved in endurance cycling