Research in the Department of Urology  

Publications (last 8 years):

1.     Bornman MS, Reif S.  Serum testosterone levels in South African men and the onset of androgen decline in aging males (LOH: late-onset hypogonadism, PADAM: partial androgen deficiency in aging men).  S Afr J Surg 2007; 45:62-64 

2.     Bornman MS, Pretorius E, Marx J, Smith E van der Merwe CF. Ultrastructural effects of DDT, DDD and DDE on neural cells of the chicken embryo model.  Environ  Environ Toxicol 2007; 328-336, 2007 

3.     McClusky LM, de Jager C, Bornman MS:  Stage-related increase in the proportion of apoptotic germ cells and altered frequencies of stages in the spermatogenic cycle following gestational, lactational and direct exposure of male rats to p-nonylphenol.  Toxicol Sci. 2007; 95(1):249-56 

4.     Dikobe AM, van Staden CW, Reif S, Bornman MS:  Deficient Testosterone Levels in Men above 45 years with Major Depressive Disorder – An Age-matched Case Control Study.  SAJMSP 2007, 13 (3): 96-100 

5.     Aneck-Hahn NH, Schulenburg GW, Bornman MS, Farias P, de Jager C:  Impaired semen quality associated with environmental DDT exposure in young men living in a malaria area in the Limpopo Province, South Africa.  J Androl 2007, 28(3):423-434 

6.     Kilian E, Delport R, Bornman MS, De Jager C:  The effects of relevant endocrine disrupting compounds on the fertility parameters in male Sprague-Dawley rats.  Andrologia 2007; 39: 128–135 

7.     Heyns CF, Bornman MS:  Men's Health in Africa. Part 1: Reproductive urogenital diseases and human immunodeficiency virus infection.  The journal of men's health & gender (jmh) 2008; 5(1):66-72 

8.     Heyns CF, Bornman MS:  Men's Health in Africa. Part 2: Non-communicable diseases, malignancies and socio-economic determinants of health.  The journal of men's health & gender (jmh) 2008;5(1):127-132 

9.     McClusky LM, Barnhoorn IEJ, van Dyk JC, Bornman MS:  Testicular apoptosis in feral Clarias gariepinus using TUNEL and cleaved caspase-3 immunohistochemistry.  Ecotoxicology and Environmental Safety 2008, 71: 41-46 

10.   Franken DR and Aneck-Hahn NH. African experience with training courses on sperm examination. Human Reproduction, 2008, 1:60-63. 

11.   Mahomed SI, Voyi K, Aneck-Hahn NH and De Jager  C.  Oestrogenicity and chemical target analysis of run off water from small-sized industries in Pretoria, South Africa. Water SA. 2008, 34(3): 357-364. 

12.   Aneck-Hahn NH, Bornman MS, de Jager C:  Preliminary assessment of estrogenic activity in water sources within a nature reserve supplying drinking water to an urban area in Gauteng, South Africa.  Afr J Aqua Sci 2008, 33(3): 249-254 

13.   Pretorius E, Bornman MS, Reif S, Oberholzer HM, Franz RC:  Ultrastructural changes of platelet aggregates and fibrin networks in a patient with renal clear cell adenocarcinoma: a scanning electron microscopy study.  Microscopy Research and Technique 2009; 72:679–683 

14.   Aneck-Hahn NH, Bornman MS, de Jager C:  Estrogenic Activity in Drinking Waters from a Rural Area in the Waterberg District, Limpopo Province, South Africa.  Water SA 2009; 35(3):245-252 

15.   Aneck-Hahn NH, de Jager C, Bornman MS:  Estrogenic Activity in Water Sources within a Nature Reserve Supplying Drinking Water to an Urban Area in Gauteng, South Africa. Water SA 2009, 35 (3):245-252 

16.   Marchand MJ, van Dyk JC, Pieterse GM, Barnhoorn IEJ, Bornman MS:  Histopathological Alterations in the Liver of the Sharptooth Catfish Clarias gariepinus from Polluted Aquatic Systems in South Africa.  Environ Toxicol 2009, 24:133-147 

17.   Eskenazi B, Anderson HA, Bornman MS, Bouwman H, Chen A, Cohn BA, de Jager C, Henshel DS, Liepzig F,  Leipzig JS, Lorenz EC, Snedeker SM, Stapleton D: The Pine River Statement: Human Health Consequences of DDT Use. Environmental Health Perspectives. The Pine River statement: Human health consequences of DDT use. Environ Health Perspect 2009, 117(9): 1359-1367 

18.   McClusky LM, Sean Patrick, Irene E. J. Barnhoorn, Jacobus C. van Dyk, Christiaan de Jager, Maria S. Bornman: Immunohistochemical study of nuclear changes associated with male germ cell death and spermiogenesis.  J Mol Hist 2009, 40:287–299. 

19.   Barnhoorn IEJ, Bornman MS, Jansen van Rensburg C, Bouwman H:  DDT residues in water, sediment, domestic and indigenous biota from a currently DDT-sprayed area. Chemosphere 2009, 77(9): 1236-1241 

20.   Van Dyk JC, Marchand MJ, Pieterse GM, Barnhoorn IEJ, Bornman MS:  Histological changes in the gills of Clarias gariepinus (Teleostei: Clariidae) from a polluted South African urban aquatic system.  Afr J Aqua Sci 2009, 34 (3): 283-291. 

21.   Mlambo SS, van Vuren JHJ, Barnhoorn IEJ, Bornman MS: Histopathological changes in the reproductive system (ovaries and testes) of Oreochromis mossambicus following exposure to DDT.  Environ. Toxicol. Pharmacol. 2009, 28(1):133-139. 

22.   de Jager C, Aneck-Hahn NH, Bornman MS, Farias P, Leter G, Eleuteri P, Rescia M, Spano M: Sperm Chromatin integrity in DDT-exposed young men living in a malaria area in the Limpopo Province, South Africa.  Human Reproduction, 2009, 24 (10):2429-2438. 

23.   Van Dyk JC, Bouwman H, Barnhoorn IEJ, Bornman MS. DDT contamination from indoor residual spraying for malaria control. Science of the Total Environment 2010, 408: 2745–2752. 

24.   Barnhoorn IEJ, Bornman, MS, Van Dyk JC, Pieterse GM: Intersex in feral indigenous freshwater Oreochromis mossambicus, from various parts in the Luvuvhu River, Limpopo Province, South Africa. Ecotoxicology and Environmental Safety 2010; 73:1537–1542. 

25.   Fatoki OS, Bornman M, Ravandhalala M, Chimuka L, Genthe B, Adeniyi A:  Phthalate ester plasticizers in freshwater systems of Venda, South Africa and potential health effects.  Water SA 2010, 36 (1): 117-126. 

26.   Bornman Riana, de Jager Christiaan, Worku Zeleke, Farias P, Reif S.  DDT and urogenital malformations in newborn boys in a malarial area.  BJU International. 106(3):405-11, 2010 Aug. 

27.   Bornman MS, IEJ Barnhoorn, L Dreyer, C de Jager, DNR Veeramachaneni:  Neoplastic lesions of the rete testis and seminiferous epithelial degeneration including microlithiasis in the common eland (Tragelaphus oryx): coincidence with fat residues of nonylphenol.  Environmental Research 2010, 100(4):327-333. 

28.   Bornman MS, Barnhoorn IEJ, de Jager C, Veeramachaneni DNR: Testicular microlithiasis and neoplastic lesions in wild eland (Tragelaphus oryx): Possible effects of exposure to environmental pollutants? Env Res 2010;110(4):327-33. 

29.   Franken DR, Aneck-Hahn N, Lombaard C and Kruger TF. Semenology training programs: 8 years’ experience. Fertility and Sterility 2010; 94(7): 2615-2619 

30.   Delport R, Bornman MS,  McIntyre U,  Oosthuizen N, Becker PJ, Aneck-Hahn NH, De Jager C: 6Changes in retinol-binding protein concentrations and thyroid homeostasis with non-occupational exposure to DDT. Environmental Health Perspectives Environ Health Perspect. 2011;119(5):647-51. 

31.   Adam A, Engelbrecht MJ, Bornman MS, Manda SO, Moshokoa E, Feilat RA. The role of the PCA3 assay in predicting prostate biopsy outcome in a South African setting.  BJU Int. 2011 108(11):1738-33. 

32.   Bornman MS (Riana), Bouwman Environmental pollutants and diseases of sexual development in humans and wildlife in South Africa: harbingers of impact on overall health?  Reprod Dom Anim 2012 47 (Suppl. 4), 327–332. 

33.   Maria (Riana) Bornman, Lawrence Schlemmer, Tertia van der Walt, Cobus van Dyk, Hindrik Bouwman.  Implications for health education and intervention strategies arising from children’s caregivers concerns following successful malaria control.  Transactions of the Royal Society of Tropical Medicine and Hygiene 2012 (106):408– 414. 

34.   Kerry Brink, Johan Jansen van Vuren, Riana Bornman.  The lack of endocrine disrupting effects in catfish (Clarias gariepinus) from a DDT sprayed area.  Environ Toxicol Pharmacol 2012; 79:256-63 

35.   Gyalpo T, Fritsche L, Bouwman H, Bornman R, Scheringer M, Hungerbühler K.  Estimation of Human Body Concentrations of DDT from Indoor Residual Spraying for Malaria Control.  Env Poll Environ Pollut. 2012 ; 169:235-41 

36.   Bouwman H, Kylin H, Sereda B, Bornman R.  High levels of DDT in breast milk: Intake, risk, lactation duration, and involvement of gender.  Environ Pollut. 2012 Nov;170:63-70. 

37.   Kerry Brink, Johan Jansen van Vuren, Riana Bornman Responses of laboratory exposed catfish (Clarias gariepinus) to environmentally relevant concentrations of p,p’-DDT.  Environ Toxicol Pharmacol 2012; 34:919–925. 

38.   Birkholtz LM, Bornman R, Focke W, Mutero C, de Jager C.  Sustainable malaria control: transdisciplinary approaches for translational applications. Malar J. 2012 Dec 26;11:431. doi: 10.1186/1475-2875-11-431. 

39.   Kruger T, Barnhoorn I, Jansen van Vuren J, Bornman R. The use of the urogenital papillae of male feral African sharptooth catfish (Clarias gariepinus) as indicator of exposure to estrogenic chemicals in two polluted dams in an urban nature reserve, Gauteng, South Africa. Ecotoxicol Environ Saf. 2013 Jan;87:98-107. 

40.   Henk Bouwman, Riana Bornman, Henk van den Berg and Henrik Kylin:   DDT: fifty years since Silent Spring.  In: Late lessons from early warnings: science, precaution, innovation.  Lessons from health hazards.  EEA Report No1/2013 ISSN 1725-9177 p272-291. 

41.   Bergman A, Heindel JJ, Kasten T, Kidd KA, Jobling S, Neira M, Zoeller RT, Becher G, Bjerregaard P, Bornman R, Brandt I, Kortenkamp A, Muir D, Drisse MN, Ochieng R, Skakkebaek NE, Byléhn AS, Iguchi T, Toppari J, Woodruff TJ. Environ Health Perspect. 2013 Apr;121(4):A104-6. doi: 10.1289/ehp.1205448 

42.   Bergman A, Andersson AM, Becher G, van den Berg M, Blumberg B, Bjerregaard P, Bornehag CG, Bornman R, Brandt I, Brian JV, Casey SC, Fowler P, Frouin H, Giudice LC, Iguchi T, Hass U, Jobling S, Juul A, Kidd KA, Kortenkamp A, et al: Science and policy on endocrine disrupters must not be mixed: a reply to a “common sense” intervention by toxicology journal editors. Environ Health 2013, 12:68. 

43.   Tindall Elizabeth A, Bornman Riana MS, van Zyl  Smit, Segone M Alpheus, Monare Richard L, Venter A Philip, Hayes M Vanessa.  BMC Urology.2013, 13:74.DOI: 10.1186/1471-2490-13-74URL:  

44.   Kristina W Whitworth, Riana Bornman, Janet I. Archer, Mwenda Kudumu, Gregory S. Travlos, Ralph T. Wilson, Matthew P. Longnecker.  Determinants of plasma DDT and DDE concentrations among South African women exposed to indoor residual spraying for malaria control.  Environ Health Perspect (submitted). 

45.   Elizabeth A Tindall, MS Riana Bornman, Smit van Zyl, Alpheus M Segone, L Richard Monare, Philip AVenter, and Vanessa M Hayes. Addressing the contribution of previously described genetic and epidemiological risk factors associated with increased prostate cancer risk and aggressive disease within men from South Africa. Research Article. BMC Urology (accepted) 

46.   Hayes V, Tindall E, Venter P, Bornman R. Genetic and epidemiological factors impacting significant increases in prostate cancer risk and aggressive disease associated with an African ancestry . BJU International. 2013.  112 , SI   (Suppl 1): 34-34. 

47.   Genthe B, Le Roux WJ, Schachtschneider K, Oberholster PJ, Aneck-Hahn NH, Charnier J. Health risk implications from simultaneous exposure to multiple environmental contaminants. Ecotoxicol Environ Safety. 2013. DOI 10.1016/j.ecoenv.2013.03.032


Technical reports (last 8 years):

1.     Bornman MS, Van Vuren JHJ, Bouwman H, de Jager C, Genthe B, Barnhoorn IEJ.  Application of Chemical and Biological Assays and Sentinel Species for Measuring EDC Activity and the Potential Health Risk in the Rietvlei Nature Reserve. Water Research Commission – K1505/1/07, 2007 (ISBN 978-1-77005-551-3). 

2.     Slabbert, JL, Venter EA, Moletsane M, van Wyk, JH, Schillack V, Aneck-Hahn NH and  Marais P. An investigation into the occurrence of steroidal hormones (estrogens) in sewage effluent using biological/biochemical and chemical techniques: 2003-2005. In Burger, A.E.C. (Ed.), 2007.  Implementation of a Research Programme for Investigating Endocrine Disrupting Contaminants in South African Water Systems. Vol. 2, Appendix 3C. WRC Report No. 1402/1/07. 

3.     Slabbert JL, Venter EA, Moletsane M, van Wyk JH, Blaise C and Aneck-Hahn NH. An investigation of the estrogenic activity in water from selected drinking water treatment processes. Report to the Water Research Commission by CSIR Natural Resources and the Environment. Report Number 1532/1/07, 2007. 

4.     Bornman MS; Barnhoorn IEJ; Aneck-Hahn NH. A pilot study on the occurence of endocrine disruptive chemicals in a DDT-sprayed area. Project No: K8/639 WRC Report No: KV220/09 ISBN No: 978-1-77005-825-5. November 2009 

5.     Genthe B, Steyn M, Aneck-Hahn NH, van Zijl C and de Jager C. A Framework for Drinking Water Quality Guidelines for Endocrine Disruptors. Water Research Commission – K5/1749/1. 2010. 

6.     MS Bornman, IEJ Barnhoorn, B Genthe.  DDT for Malaria Control: Effects in Indicators and Health Risk.  Report to the Water Research Commission Report No. 1674/1/09; ISBN 978-1-77005-915-3, February2010. 

7.     De Jager C; Aneck-Hahn NH; Barnhoorn IEJ; Pieters R; van Wyk JH; van Zijl C; Bornman MS; 2011/01/01;  Detection and analysis of endocrine disrupting compounds in water   Research Report No.1816/1/10 

8.     Pletsche BI, Mwila K, van Zijl MC, Aneck-Hahn NH, van Dyk JS and Burton M. Rapid enzymatic detection of organophosphorus and carbamate pesticides in water.  WRC Report no 1902/1/11, 2011 

9.     Oberholster PJ, Aneck-Hahn NH, Ashtona PJ, Botha A-M, BrownJ, Dabrowski JM, de Klerk AR, de Klerk LP,  Genthe B, Geyer H, Hall G, Hill L, Hoffman A,  Kleynhan CJ, Lai J, le Roux W, Luus-Powell W, Masekoameng E, McMillan P, Myburgh J, Schachtschneider K, Somerset V, Steyl J,  Surridge AKJ, Swanevelder ZH, van Zijl MC, Williams C, Woodborne S. Risk assessment of pollution in surface waters of the upper Olifants river system: implications for aquatic ecosystem health and the health of human users of water. CSIR report number: CSIR/NRE/WR/IR/2011/0041/B 

10.   de Jager C; Aneck-Hahn NH; Barnhoorn IEJ; Bornman MS; van VurenJHJ; Burger AEC; Swemmer A; van Zijl C; van Wyk S; Jonker M.  Endocrine Disrupting Chemical (EDC) Activity and Health. Effects of Identified Veterinary Growth Stimulants in Surface and Ground Water.  WRC Research Report No.1686/1/11; 2011/05/01. Available at

 11.   Barnhoorn IEJ, Bornman MS, van Dyk JC, Genthe B and Pieterse GM.  Edibility of Selected Freshwater Fish from the Rietvlei Dam. WRC Report No. KV 281/11 ISBN 978-1-4312-0161-7. Mar 2013.
DeJager C, Aneck-Hahn NH, Swart P, Truebody B, Van Zijl MC. Estrogenic activity and endocrine disrupting chemical (EDC) status in water obtained from selected distribution points in Pretoria and Cape Town. WRC Report No KV317/13 


Published by Leonore Jordaan

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