Matrix assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF) mass spectrometry (MS) is widely considered as the reference method for the rapid and inexpensive identification of microorganisms in routine laboratories. This automatic technique is a reference method for identifying microorganisms such as bacteria, mycobacteria and fungi. Routine diagnostics is currently carried out by the UP diagnostics Platform. The division of Plant Pathology currently hosts the Bruker MALDI Biotyper and the MALDI Biotyper Sirius RUO platform, co-funded by the National Research Foundation (NRF).
How to submit a sample:
Samples are accepted on week days from Monday to Friday, 09h00-16h00.
Samples should be labelled with a maximum of 4 characters including letters, commas and numbers (e.g. E1.1/ E2/ EF1 etc.) or alternatively numerically (e.g. if you have 10 samples you may label them 1 to 10). Labels should not include asterisks and exponential/scientific notation.
Before submitting your samples please make sure:
This may assist in minimizing the chances of a "poor identification", "No Organism Identification Possible" or a "no identification (No peaks found)" result.
Please contact us when you are ready to submit a sample; you will be sent a:
Once we have received a signed quotation you will be sent an invoice for payment. Samples can then be shipped. We would like to request that samples be shipped in an upright position to prevent spillage, which may lead to sample loss and ethanol removing the sample label.
Note that no reports will be issued until proof of payment has been received.
The shipping address and contact details for samples is:
Department of Plant and Soil Sciences
Room 2-16, Level 2, Agricultural Science Building
University of Pretoria, Private Bag X20
Hatfield 0028, South Africa
Office: 012 420 4588
Mobile: 084 364 1306
Email: [email protected]
The NRF and DSI logos according to the current branding policy should appear on all reports, presentations, publications and webpages that arise from any material using data generated from the MALDI-TOF biotyper.
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