Yearbooks

Genetic manipulation of microbes 364


 
Module code MBY 364
Qualification Undergraduate
Faculty Faculty of Natural and Agricultural Sciences
Module content

Isolation of clonable DNA (genomic libraries, cDNA synthesis) cloning vectors (plasmids, bacteriophages, cosmids) plasmid incompatibility and control of copy number. Ligation of DNA fragments, modification of DNA end and different ligation strategies. Direct and indirect methods for the identification of recombinant organisms. Characterization (polymerase chain reaction, nucleic acid sequencing) and mutagenisis of cloned DNA fragments. Gene expression in Gram negative (E.coli) Gram positive (B.subtilis) and yeast cells (S.cerevisea). Use of Agrobacterium and baculoviruses for gene expression in plant and insect cells respectively. Applications in protein engineering, diagnostics and synthesis of useful products.

Module credits 18.00
Prerequisites BCM 251, GTS 251 and MBY 251 or permission from the HOD.
Contact time 1 practical per week, 2 lectures per week
Language of tuition Module is presented in English
Department Biochemistry, Genetics and Microbiology
Period of presentation Semester 2

The information published here is subject to change and may be amended after the publication of this information. The General Regulations (G Regulations) apply to all faculties of the University of Pretoria. It is expected of each student to familiarise himself or herself well with these regulations as well as with the information contained in the General Rules section. Ignorance concerning these regulations and rules will not be accepted as an excuse for any transgression.

Copyright © University of Pretoria 2019. All rights reserved.

FAQ's Email Us Virtual Campus Share