Nomenclature: enzyme nomenclature and classification. Specificity and mechanisms: the active site, mechanisms of catalysis and examples of specific enzyme mechanisms, e.g. lysozyme and carboxypeptidase A. Advanced enzyme kinetics, Cleland nomenclature and multi-substrate reactions. Allosteric enzymes: models by Koshland, Hill and Monod. Ligands binding to proteins. Problems and answers: tutorials of problems and answers based on above concepts. Integration of metabolism; hormones and second messengers; cell signalling; a case study in connectivity among metabolic pathways and their regulation, in for example diabetes and starvation. Inhibitors of angiotensin converting enzyme (ACE). RNA as enzymes. Applications of enzymes in food and cosmetics industries and in clinical pathology assays as biomarkers of diseases and toxic responses. Elucidation of metabolic pathways.
Practical sessions cover tutorials on calculations, isolation of an enzyme, determination of pH and temperature optimum, determination of Km and Vmax, enzyme activation, enzyme inhibition, purification table and final report, oral defense of report.