Prof Don A Cowan

Professor

 

don.cowan [at] up.ac.za
012 420 5873 or 012 420 6929 
Office:   NW II building, rm 3-12

http://www.up.ac.za/microbial-ecology-and-genomics

 

Qualifications

PhD (U. Waikato, NZ) 

Don Cowan was educated (BSc, MSc, PhD) at the University of Waikato (New Zealand) and completed a 4-year period of postdoctoral research under the supervision of Professor Roy Daniel before moving to a Lectureship at University College London (UK) in 1985. After 16 years in London he was appointed to the Chair of Microbiology and Head of the Department of Biotechnology at the University of the Western Cape (RSA), where he established the Institute for Microbial Ecology and Metagenomics. In 2012 he moved to the University of Pretoria where he is the Director of both the Genomics Research Institute and the Centre for Microbial Ecology and Genomics.

Don’s research is in the fields of microbial ecology, microbial genomics and applied microbiology, where he and his team use modern ‘omics’ methods to understand the diversity and function of microorganisms in different environments. Much of his research focuses on the microbiology of extreme environments, including hot (Namib) and cold (Antarctic) desert soils, but he also supervises numerous other microbiomics projects.  He collaborates with many other research laboratories in South Africa and across the world.

Don has published over 300 research papers, review articles and book chapters and sits on the Editorial Boards of 12 international journals.  He has an h-index of 38 (Scopus).

In the course of his career, Don has trained and graduated 32 PhD students and 42 MSc students.  He currently supervises a research team of 3 research fellows, 10 postdoctoral researchers, 8 PhDs and 6 MScs.

He also holds the post of Adjunct Professor at the University of Waikato (NZ), was elected as a Fellow of the Royal Society of South Africa in 2007, as a Member of the Academy of Sciences of South Africa in 2008, and as an Honorary Fellow of the Royal Society of New Zealand in 2009. He was awarded the University of the Western Cape Vice-Rector’s Award for Research Excellence in 2008 and the South African Society for Microbiology Medal for Research Excellence in 2009.  He is the immediate past-President of the Royal Society of South Africa. He was awarded an NRF A-rating in 2013 and received the National Science and Technology Forum Capacity Development award in 2014.  In April 2015 he received the University of Pretoria’s highest research award, the Chancellor’s Medal.

 

Teaching subjects

Have taught: Proteins and Enzymes; Industrial Microbiology: Microbial Ecology.

 

Research focus

Areas of research include

Microbial ecology, functional metagenomics, bacterial genomics, microbial stress responses; applied microbiology and enzymology; extremophiles; microbial biocatalysis.

http://www.up.ac.za/microbial-ecology-and-genomics


Specific research projects include

Metagenomics of Antarctic cold desert ecosystems

Genomics and and comparative genomic of Geobacillus

Microbial ecology of Namib Desert soil ecosystems

Microbial community structure of crop plant rhizospheric soils

Landscape-scale surveys of sub-Saharan soil microbial communities

Novel microbial stress response and adaptation genes

Metaviromics of desert ecosystems

Gene discovery for new biotransformation enzymes

The structure-function of novel lignocellulosic degrading enzymes 

 
 

Technical Assistants and Research Fellows

Name

Position

Location

Dr Angel Valverde

Research Fellow

UP

Dr Jean-Baptiste Ramond

Research Fellow

UP

Dr Marnie Potgieter

Research Fellow

UP

Dr Surendra Vikram

Post Doc

UP

Dr Jasmin Mertens

Post Doc

UP

Dr Gilbert Kamgan

Post Doc

UP

Dr Pedro Lebre

Post Doc

UP

Dr Riaan Rifkin

Post Doc

UP

Dr Habibu Aliyu

Post Doc

UP

Dr David Reed

Post Doc

UP

Dr Sandra Heinzelmann

Post Doc

UP

Dr Carlos Leon-Sobrino

Post Doc

UP

Dr Laura Martinez

Post Doc

UP

 

Postgrad students

Name

Degree

Location

Supervisor/co-Supervisor

Tshifhiwa Mamphogoro

PhD

CSIR

S

Philippa Fanzini

PhD

UP

S

Jonathan Botha

PhD

UP

S

Leticia Mosina

PhD

UP

S

Jane Raphela

PhD

CSIR

S

N Mahaye

PhD

CSIR

S

Fiyin Adesioye

PhD

UP

S

Tanya Oberholster

PhD

UP

S

Riegardt Johnson

MSc

UP

S

Pieter Bouwman

MSc

UP

S

Khaoli Xaba

MSc

UP

S

Andries van der Walt

MSc

UP

Co-S

Storme de Scally

MSc

UP

Co-S

Janine Baxter

MSc

UP

S

Michael Clayton

MSc

UP

Co-S

Shamara Polliack

MSc

UP

Co-S

Nadine Koen

MSc

UP

Co-S

 

Community Involvement

  • Immediate-past President, Royal Society of South Africa
  • Applied and Environmental Microbiology [IF 3.83], 2012 – date
  • Biotechnology Journal [IF = 3.0]. 2005 - date
  • Biotechnology Letters [IF = 1.85]. 1999 – date.
  • Cell Biochemistry and Function [IF 1.77]. 2008 – date
  • Extremophiles [IF = 2.94]. 1996 – date.
  • Frontiers in Microbiology [Associate Editor], 2013 – date
  • Journal of Applied Microbiology [IF = 2.34]/Letters in Applied Microbiology [IF = 1.62]. 2002  - date.
  • Life (http://www.mdpi.com/journal/life/editors); 2014 - date
  • Microbial Cell Factories [IF = 3.55]. 2005 – date
  • Microbial Genomics, 2015 - date
  • Microorganisms, 2016 –
  • PLoS ONE [IF = 3.73], 2014 – date    
  • Trends in Biotechnology [IF = 9.15]. 1995 – date

Society Memberships

The Royal Society of South Africa  (Immediate Past President) 
Society for General Microbiology
International society for Extremophiles  (Council Member)
International Society for Microbial Ecology 
SA Society for Biochemistry and Molecular Biology  (Past President) 

International Society for Functional Metagenomics (Founder and Committee Member)
 

Publications
List: 2015 – 2016 only (for complete list, see www.up.ac.za/CMEG)

 
BOOK CHAPTERS
  • Cowan, DA, Burton, SB (2002) Archaea in Biotechnology. Encyclopaedia of Environmental Microbiology. Wiley, Publ. pp. 259-276
  • Daniel, RM, J.F. Holden, R. van Eckert, J. Truter and D.A. Cowan (2004) The stability of biomolecules and the implications for life at high temperatures. In Subsurface Biosphere at Mid-Ocean Ridges (W.S.D. Wilcock, C. Cary, E. DeLong, D.S. Kelley, and J.A. Baross, Eds. American Geophysical Union Monograph, Washington, D.C., pp. 264-289.
  • Cowan, DA, Ah Tow, L, Smith, JJ, Cary, C, Moodley, K. (2005) Microbial molecular ecology of Antarctic mineral soil biotopes. In Antarctica; Global Laboratory for Scientific and International Collaboration, A. Tan, Z Yasin, M Mansor, eds., Academy of Sciences Malaysia, Kuala Lumpur, pp. 113-118.
  • Sjöling, S. Stafford, W, Cowan, DA (2006) Soil metagenomics: Exploring and exploiting the soil microbial gene pool. Ch. 16 in Modern Soil Microbiology, 2nd Edn. van Elsas, JD, Jansson, J, Trevors, JT, Eds., CRC Press, Taylor & Francis, pp. 241 – 266.
  • Russell, NR and Cowan, DA. (2006) Methods for the handling of psychrophiles microorganisms. Ch. in Methods in Microbiology. Vol. 35, Oren, A, & Rainey, F, eds., Elsevier Publ., pp371-393 [ISBN 978-0-12-521537-4].
  • Cowan, DA, Casanueva, A, Stafford, W. (2007) Ecology and biodiversity of cold-adapted organisms. Ch. 9 in Physiology and Biochemistry of Extremophiles. Gerday, C., Glansdorff, N., eds. ASM Press, Washington DC, pp. 119-133.
  • Cowan, DA and Stafford, W. (2007) Metagenomic methods for determining active microorganisms and genes in bioremediation and biotransformation processes. Ch. 58 in ASM Manual of Environmental Microbiology, 3rd Edition. Editors: CJ Hurst, RL. Crawford, JL. Garland, DA Lipson, AL Mills, LD Stetzenbach., ASM Press, New York. 1310 pp. ISBN: 978-1-55581-379-6
  • Daniel, R.M., Danson, M.J., Hough, D.W., Lee, C.K., Peterson, M.E. and Cowan, D.A. (2008) Enzyme stability and activity at high temperatures. Ch. 17 in Anatomy & Physiology of Extremophilic Proteins, Nova Science, Eds. Thomas, T, Sidiqui, S. pp. 1-34. ISBN 978-1-60456-019-0
  • Cowan, DA, Sjoling, S, Stafford, WHL, Rohr, LM, Heath, C. (2008) Metagenomics in Agribiotechnology. In Proceedings of the Asia Pacific Conference on Plant Tissue Culture and Agrobiotechnology (APaCPA 2007). Aimst University, Bedong, Malaysia.
  • Sjoling, S, Cowan, DA. (2008) Metagenomics – microbial community genomes revealed. Ch. 20 in Psychrophiles: from Biodiversity to Biotechnology. Margesin, R.; Schinner, F.; Marx, J.-C.; Gerday, C. (Eds.) 462 pp. ISBN: 978-3-540-74334-7
  • Berjak, P, Cowan, DA, Cowling, R, Pammenter N, Scholes, R. (2009) Biological Sciences. Ch. 5 in The State of Science in South Africa. Diab, R, Gevers, W., Eds, ASSAf Publ., pp. 133-163.
  • Kirby, BM, Vengadajellum, CJ, Burton, SG, Cowan, DA. (2010) Anthropogenically-created habitats - coal, coal mines and spoil heaps. In: Handbook of Hydrocarbon and Lipid Microbiology, Timmis, Kenneth N. (Ed.), Springer Heidelberg, Vol 3, pp. 2277-2292.
  • Cowan, DA, Tuffin, IM, Robb, F. (2010) ‘‘Sequence First, Ask Questions Later’’: The Impact of Next Generation Omics on the Discovery of Novel Microbial and Lipid Hydrocarbon Metabolism. In: Handbook of Hydrocarbon and Lipid Microbiology, Timmis, Kenneth N. (Ed.), Springer Heidelberg, Vol 4, pp. 3367-3374.
  • Cowan, DA, Khan, N, Heath, C, Mutondo, M. (2010) Microbiology of Antarctic terrestrial soils and rocks. Ch. 1 in Polar Microbiology: The Ecology, Biodiversity and Bioremediation Potential of Microorganisms in Extremely Cold Environments. Atlas, R, Aislabie, J, Bej, A, Eds. CRC Press, pp. 1-30
  • Cowan, DA, Kirby, BM, Meiring, TL, Ferrer, M, Guazzaroni, M-E, Golyshina,, OV, Golyshin, PN (2010) Enzymes from Extreme Environments Ch. 4. In Manual of Industrial Microbiology and Biotechnology, 3rd Edition, Baltz, RH, Davies, JE, Demain, AL, Eds. ASM Press, ISBN: 978-1-55581-512-7
  • Meiring, T.L, Mulako, I, Tuffin, M, Meyer, Q, Cowan, DA. (2010) Retrieval of full length functional genes using subtractive hybridization magnetic bead capture. Ch. 20 in: Metagenomics: Methods and Protocols, Vol. 668 of Methods in Molecular Biology, WR. Streit and R. Daniel (Eds.): Humana Press Inc., Totowa NJ, USA. Pp 287-298. ISBN: 978-1-60761-822-5
  • Meiring, TL, Bauer, R, Scheepers, I, Ohloff, C, Tuffin, IM, Cowan, DA. (2011) Metagenomics and beyond: current approaches and integration with complementary technologies. Ch. 1 in Metagenomics: Current Innovations and Future Trends. Marco, D., Ed., Horizon Press, Publ. pp. 1 – 19.
  • Bauer, R, Mutondo, M, Huddy, RJ, Tuffin, IM, Cowan, DA. (2011) Metagenomic gene discovery. Ch. 15 in Metagenomics and its Applications in Agriculture, Biomedicine and Environmental Studies. RW Li, Ed. Nova Science Publ. pp. 287-320.
  • Grant, WD, Pagaling, E, Marquez, C, Gutierrez, C, Cowan, DA, Ma, Y, Jones, BE, Ventosa, A, Heaphy, S. (2011) The Hypersaline Lakes of Inner Mongolia. Ch. 4 in Halophiles and Hypersaline Environments, A. Ventosa et al. (eds.), Springer-Verlag Berlin, pp. 65-107.
  • Taylor, MP, Van Zyl, L, Tuffin, M, Cowan, DA. (2011) Extremophiles and biotechnology: How far have we come? Ch. 1. In Extremophiles: Microbiology and Biotechnology. Anitori, RP, ed. Caister Academic Press, Norwich, pp. 1-23
  • Kirby, BM, Le Roes-Hill, M, Cary, SC, Burton, SG, Tuffin, IM, Cowan, DA. (2011) Actinobacterial diversity associated with Antarctic Dry Valley mineral soils. Ch. 13 in Handbook of Molecular Microbial Ecology II: Metagenomics in Different Habitats, Frans J. de Bruijn, Ed., John Wiley & Sons, New Jersey. ISBN 978-0-470-64719-6
  • Taylor, MT, Ramond, J-B, Tuffin, IM, Burton, SG, Eley, K, Cowan, DA. (2011) Mechanisms and Applications of Microbial Solvent Tolerance. Ch. 6 in Microbial stress tolerance for biofuels: systems biology. Microbiology Monographs, vol. 22, L. Liu, Ed., Springer, London. pp. 177-208
  • Kirby, BM, Barnard, D, Tuffin, IM, Cowan, DA. (2011) Ecological distribution of microorganisms in terrestrial, psychrophilic habitats. In: Extremophiles Handbook., Horikoshi, K.; Antranikian, G.; Bull, A.T.; Robb, F.T.; Stetter, K.O. (Eds.) pp. 839-859.
  • Kirby, BM, Easton, S, Tuffin, IM, Cowan, DA. (2012) Bacterial Diversity in Polar Habitats. Ch. in Polar Microbiology: Life in a deep freeze. Miller, RV, Whyte, LG., Eds. ASM Press. ISBN 978-1-55581-604-9. 312pp.
  • Cowan, D., Tuffin, M., Mulako, I., Cass, J. (2012) Life in Terrestrial Hydrothermal Environments. Ch. in Life in Extremes: Environments, Organisms and Strategies for Survival. Bell, E., Ed., CABI Press. 576 pp. ISBN 978 1 84593 814 7
  • Taylor, MP, Bauer, R, Mackay, S, Tuffin, IM, Cowan, DA. (2012) Extremophiles and their application to biofuel research. In: Sustainable Resources and Biotechnological Implications. OV Singh, Ed, Wiley. In Press
  • De Maayer, P, Valverde, A, Cowan DA. (2014) The current state of metagenomic analysis. Ch. 10, in Genomic Analysis: Current Protocols and Applications, Poptsova MS, Ed. Caister Academic Press, Norfolk, UK, pp. 183 – 220.
  • Valverde, A, De Maayer, P, Cowan DA. (2014) Microbial community profiling: current approaches and future trends. Ch. 6, In Bioinformatics and Data Analysis in Microbiology, O. Tastan-Bishop, eds. Caister Academic Press, pp. 143-162. ISBN: 978-1-908230-39-3
  • Cowan, DA. (2014) Introduction. Ch. 1. In Antarctic terrestrial microbiology. Cowan, DA, Ed. Springer-Verlag, Berlin, pp. 1-8.
  • Makhalanyane, T, Pointing, S, Cowan, DA. (2014) Lithic soil communities. Ch. 9 in Antarctic Terrestrial Microbiology. Cowan, DA, Ed. Springer-Verlag, Berlin, pp. 163-180.
  • Berger E, Ferreras E, Taylor MP, Cowan DA (2015) Extremophiles and their use in biofuel synthesis. Ch. In Industrial Biocatalysis. Vol. 1. Grunwald P, Ed., Pan Stanford Publ. Singapore. pp 239 - 281  ISBN 978-981-4463-89-8.
  • Smart M, Huddy RJ, Cowan DA, Trindade IM (2016) Liquid-phase multiplex high-throughput screening of metagenomic libraries using p-nitrophenyl-linked substrates for accessory lignocellulosic enzymes. Ch. In Metagenomics: Methods and Protocols. 2nd Edn., Streit W, Daniel R, Eds. Springer. In Press




PAPERS [Values in brackets: IF/#Citations: data from Google Scholar®]

  • Fernandez-Lafuente, R, Guisan, JM, Ali, S, Cowan, DA (2000) Immobilization of functionally unstable catechol-2,3-dioxygenase greatly improves operational stability. Enzyme Microb. Technol. 26:268-573. [2.3/10]
  • Cowan, DA (2000) Microbial genomes-the untapped resource. Trends Biotechnol. 18:14-16. [9.6/69]
  • Graham, D, Barfield, D, Pereira, RA, Cowan, DA (2000) Nitrile transformation studies using free and immobilised cells of a thermophilic Bacillus sp. Enzyme Microb. Technol. 26:368-373. [2.3/36]
  • Daniel, RM, Cowan, DA (2000) Biomolecular stability and life at high temperatures. Cell. Mol. Life Sci. 57:250-64 [7.0/91]
  • Huneke, F-U, Bailey, D, Nucci, R, Cowan, DA (2000) Sulfolobus solfataricus ƒÒ-glucosidase catalysed synthesis of alkyl glycosides in organic solvents. Biocatal. Biotrans. 18:291-299. [6]
  • Sjoling, S, Cowan, DA (2000) Presence of human-specific enteric micro-organisms in current and historic field camp sites. Polar Biol. 23:644-650. [4]
  • Cowan, DA (2000) Using your neighbours genome! Nature 407:466-467.
  • Atkinson, T, Cairns, S, Cowan, DA, Danson, MJ, Hough, DW, Johnson, DB, Norris, P, Raven, N, Robson, R, Robinson, C, Sharp, RJ (2000) A microbiological survey of Montserrat Island hydrothermal biotopes, Extremophiles 4:305-314. [2.2/31]
  • Baker, GC, Gaffar, S, Cowan, DA Suharto, AR (2001) Microbial community analysis of Indonesian hot-springs. FEMS Microbiol. Lett. 200:103-109 [2.0/31]
  • Burton, S, Cowan, DA, Woodley, JM (2002) The search for the ideal biocatalyst. Nature Biotechnol. 30:35-46 [31.1/161]
  • Burton, SG & Cowan, DA (2002) Development of biotechnology in South Africa. Electron. J. Biotechnol. 5 :33-36. [0.9/2]
  • Bustard, MT, Whiting, S., Cowan, DA, Wright, PC (2002) Biodegradation of high concentration solvents by a solvent tolerant thermophile, Bacillus pallidus. Extremophiles 6:319-323. [2.2/21]
  • Wilkinson, D, Jaenicke, T, Cowan, DA (2002) Efficient cloning of DNA from environmental sources. Biotechnol. Lett. 24:155-161. [1.8/22]
  • Cowan, D.A., Mamais, A. Sheppard, D, Russell, N (2002) Antarctic Dry Valley mineral soils contain unexpectedly high levels of microbial biomass. Extremophiles 6:431-436. [2.2/55]
  • Cowan, DA, Cameron, R, Tsekoa, T (2003) Comparative biology of thermophilic and mesophilic nitrile hydratases, Adv. Appl. Microbiol. 52:123-158. [3.9/15]
  • Baker, G, Ah Tow, L, Cowan, DA (2003) PCR-based detection of non-indigenous micro-organisms in ‘pristine’ environments. J. Microbiol. Methods 53:157-164. [2.0/8]
  • Sjoling, S, Cowan, DA (2003) High 16S rDNA bacterial diversity in glacial meltwater lake sediment, Bratina Island, Antarctica, Extremophiles 7:275-282. [2.2/39]
  • Ah Tow, L, Cowan, DA (2003) Non-specificity of Staphylococcus generic primers. Microbiology 149:1605-1607. [3.0/1]
  • Baker, G, Smith, JJ, Cowan DA (2003) Review and re-analysis of Domain-specific 16S primers, J. Microbiol. Methods 55:451-455. [2.0/409]
  • Cowan DA, Arslanoglu, A., Burton, SG, Baker, GC, Cameron, RA, Smith, JJ, Meyer, Q (2004) Metagenomics, gene discovery and the ideal biocatalyst. Biochem. Soc. Trans. 32:298–302. [3.4/28]
  • Baker, GC, Cowan, DA (2004) 16S rDNA primers and the unbiased assessment of thermophile diversity. Biochem. Soc. Trans. 32:218-221. [3.4/17]
  • Cowan, DA (2004) The upper limit of life - how far can we go? Trends Microbiol. 12:58-60. [7.5/11]
  • Cowan, DA, Ah Tow, L (2004) Endangered Antarctic microbial communities. Ann. Rev. Microbiol. 58, 649-690. [12.4/58]
  • Tsekoa, T, Sayed, M, Sewell, T, Cowan DA. (2004) Preliminary structural analysis of a thermostable nitrile hydratase. S.A. J. Sci. 100, 488-490. [0.5/1]
  • Cameron, RA, Sayed, M, Cowan, DA (2005) Molecular analysis of the nitrile catabolism operon of the thermophile Bacillus pallidus RAPc8. Biochim. Biophys. Acta. 1725, 35-46. [2.5/14]
  • Cowan, DA, Meyer, Q, Stafford, W., Muyanga, S., Cameron, RA, Wittwer P. (2005) Metagenomic gene discovery: past, present and future. Trends Biotechnol. 23:321-329. [9.6/129]
  • Ah Tow, L, Cowan, DA. (2005) Dissemination and survival of non-indigenous bacterial genomes in pristine Antarctic environments. Extremophiles 9:385-389. [2.3/5]
  • Xue, Y, Fan, H, Ventosa, A, Grant, WD, Jones, B, Cowan, DA, Ma, Y. (2005) Haloalkalicoccus tibetense gen. nov., sp. nov. a novel genus of haloalkaliphilic archaeon. Int. J. System. Evol. Microbiol. 55:2501-2505. [2.1/15]
  • Stafford, WHL, Baker, GC, Brown, SA, Burton SG, Cowan DA. (2005) Bacterial diversity in the rhizosphere of Proteaceae species. Environ. Microbiol. 7:1755-1768. [5.5/11]
  • Grant, S, Grant, W, Cowan, DA, Jones, B, Ma, Y, Ventosa, A, Heaphy, S. (2006) Identification of eukaryotic open reading frames in metagenomic cDNA libraries made from environmental samples. Appl. Environ. Microbiol. 72:135-43. [3.8/38]
  • Carrasco, IJ, Marquez, MC, Yanfen, X, Ma, Y, Cowan, DA, Jones, BE, Grant WD, Ventosa, A. (2006) Gracilibacillus orientalis sp. nov., a novel moderately halophilic bacterium isolated from a salt lake in Inner Mongolia, China. Int. J. System. Evol. Microbiol. 56:599-604 [1.9/16]
  • Castillo AM, Gutierrez MC, Kamekura M, Ma Y, Cowan DA, Jones BE, Grant WD, Ventosa, A. (2006) Halovivax asiaticus gen. nov. sp. nov., a novel extremely halophilic archaeon isolated from Inner Mongolia, China. Int. J. System. Evol. Microbiol. 56:765-770. [1.9/17]
  • Smith, JJ, Ah Tow, L, Stafford, W, Cary, C, Cowan, DA (2006) Bacterial diversity in three different Antarctic cold desert mineral soils, Microb. Ecol. 51:413-421. [2.9/57]
  • Xue Y, Zhang X, Zhou C, Zhao Y, Cowan DA, Heaphy S, Grant WD, Jones BE, Ventosa, A, Ma Y. (2006) Caldalkalibacillus thermarum gen. nov., sp. nov., a novel alkalithermophilic bacterium from a hot spring in China. Int. J. Syst. Evol. Microbiol. 56:1217-1221. [1.9/2]
  • Castillo AM, Gutierrez MC, Kamekura M, Xue Y, Ma Y, Cowan DA, Jones BE, Grant WD, Ventosa A. (2006) Halostagnicola larsenii gen. nov., sp. nov., an extremely halophilic archaeon from a saline lake in Inner Mongolia, China. Int. J. System. Evol. Microbiol. 56:1519-1524. [2.1/11]
  • Dürr, R, Vielhauer, O, Burton, SG, Cowan, DA, Puñal, A, Brandão, PFB, Bull AT, Syldatk, C. (2006) Distribution of hydantoinase activity in bacterial isolates from geographically distinct environmental sources. J. Mol. Cataly. B. Enzymic. 39:160-165. [2.3/3]
  • Castillo AM, Gutierrez MC, Kamekura M, Xue Y, Ma Y, Cowan DA, Jones BE, Grant WD, Ventosa, A. (2006) Halorubrum orientale sp. nov., a novel halophilic archaeon isolated from Lake Ejinor, Inner Mongolia, China. Int. J. System. Evol. Microbiol. 56:2559-63. [1.9/8]
  • Castillo AM, Gutierrez MC, Kamekura M, Xue Y, Ma Y, Cowan DA, Jones BE, Grant WD, Ventosa, A. (2006) Natrinema ejinorense sp. nov., isolated from a saline lake in Inner Mongolia, China. Int J Syst Evol Microbiol. 56:2683-2687. [1.9/3]
  • Agakar, VB, Kimini, SH, Cowan, DA, Sayed, MSF-R, Sewell, TB. (2006) The quaternary structure of the amidase from Geobacillus pallidus RAPc8 is revealed by its crystal packing. Acta Cryst. F. 62:1174-1178. [0.5/6]
  • Meyer, QC, Burton, SB, Cowan, DA (2007) Subtractive hybridization magnetic bead capture: A new technique for the recovery of full length ORFs from the metagenome. Biotechnol. J. 2:36-40 [(2.1)/11]
  • Makhongela, HS, Glowacka, A, Agarkar, VB, Sewell, BT, Weber, B, Cameron, RA, Cowan, DA and Burton, SG. (2007) A novel thermostable nitrilase superfamily amidase from Geobacillus pallidus showing acyl transfer activity. Appl. Microbiol. Biotechnol. 75:801-811. [3.3/11]
  • Márquez, C, Carrasco, IJ, Xue, Y, Ma, Y, Cowan, DA, Jones, BE, Grant WD, Ventosa, A. (2007) Aquisalimonas asiatica gen. nov., sp. nov., a moderately halophilic bacterium isolated from an alkaline, saline lake in Inner Mongolia, China. Int. J. System. Evol. Microbiol. 57:1137-1142. [1.9/3]
  • Castillo, AM, Gutierrez, MC, Kamekura, K, Ma, Y, Cowan, DA, Jones, BE, Grant WD, Ventosa, A. (2007) Halovivax ruber sp. nov., a novel extremely halophilic archaeon isolated from Inner Mongolia, China. Int. J. System. Evol. Microbiol. 57:1024-1027. [1.9/3]
  • Olaniran, AO, Stafford, WHL, Cowan, DA, Pillay, D, Pillay, B. (2007) Microbial community profiling in cis- and trans-dichloroethene enrichment systems using denaturing gradient gel electrophoresis. J. Microbiol. Biotechnol. 17:560-570. [1.2/4]
  • Rashamuse, KJ, Burton, SG, Stafford, W, Cowan, DA. (2007) Molecular characterization of a novel family VIII esterase from Burkholdaria multivorans UWC10. J. Mol. Microbiol. Biotechnol. 13:181-188 [2.6/2]
  • Gutiérrez, MC, Castillo, AM, Kamekura, M, Xue, Y, Ma, Y, Cowan, DA, Jones, BE, Grant, WD, Ventosa, A. (2007) Halopiger xanaduensis gen. nov., sp. nov., an extremely halophilic archaeon isolated from a saline lake in Inner Mongolia, China. Int. J. System. Evol. Microbiol. 57:1402-1407. [1.9/9]
  • Silva-Stenico, E, Vengadajellum, CJ, Janjua, HJ, Harrison, STL, Burton, SG, Cowan, DA. (2007) Degradation of low rank coal by Trichoderma atroviride ES11. J. Indust. Microbiol. Biotechnol. 34:625-631. [1.8/3]
  • Pagaling, E., Haigh, R., Grant, WD, Cowan, DA, Jones, B., Ma, Y., Ventosa A., Shaun Heaphy. (2007) Sequence analysis of an archaeal virus isolated from a hypersaline lake in Inner Mongolia, China. BMC Genomics, 8:410-416 [4.2/28]
  • Carrasco, IJ, Márquez, MC, Xue, Y, Ma, Y, Cowan, DA, Jones, BE, Grant, WD, Ventosa, A. (2007) Bacillus chagannorensis sp. nov., a moderate halophile from a soda lake in Inner Mongolia, China. Int. J. System. Evol. Microbiol. 57:2084-2088. [2.1/8]
  • Carrasco, IJ, Márquez, MC, Xue, Y, Ma, Y, Cowan, DA, Jones, BE, Grant, WD, Ventosa, A. (2007) Salsuginibacillus kocurii gen. nov., sp. nov., a moderately halophilic bacterium from soda lake sediment. Int. J. System. Evol. Microbiol. 57: 2381-2386. [2.1/7]
  • Rashamuse, KJ, Burton, SG, Cowan, DA. (2007) A novel recombinant ethyl ferulate esterase from Burkholdaria multivorans. J Appl. Microbiol. 103:1610-1620. [2.4/6]
  • Carrasco, IJ, Márquez, MC, Xue, Y, Ma, Y, Cowan, DA, Jones, BE, Grant, WD, Ventosa, A. (2007) Halorubrum ejinorense sp. nov., isolated from Lake Ejinor, Inner Mongolia, China. Int. J. System. Evol. Microbiol. 57:2538-2542. [2.1/6]
  • Kimani, SW, Agarkar, VB, Cowan, DA, Sayed, MF-R, Sewell, BT. (2007) The crystal structure of an aliphatic amidase from Geobacillus pallidus RAPc8: evidence for a fourth nitrilase catalytic residue. Acta Cryst. D. 63:1048-1058. [6.3/12]
  • Cowan, DA, Casanueva, A. (2007) Stability of ATP in Antarctic desert soils. Polar Biology, 30:1599-1603. [1.4/5]
  • Porter, D., Roychoudhury, AN, Cowan, DA. (2007) Dissimilatory sulphate reduction in hypersaline coastal pans: Part 1. Activity across a salinity gradient. Geochim Cosmochim. Acta. 71:5102-5116 [4.1/11]
  • Wood SA, Rueckert A, Cowan DA, Cary SC. (2008) Aquatic cyanobacteria, important contributors to edaphic cyanobacterial diversity in the Dry Valleys of Eastern Antarctica? ISME J. 2:308-320 [6.2/29]
  • Oboirien, BO, Burton, SG, Cowan, DA, Harrison, STL. (2008) The effect of the particulate phase on coal biosolubilisation mediated by Trichoderma atroviride in a slurry bioreactor. Fuel Processing Technol. 89:123-130. [2.8/4]
  • Casanueva, AI, Galada, N, Baker, GC, Grant, WD, Heaphy, S, Jones, BW, Ma Y, Ventosa, A, Blamey A, Cowan, DA. (2008) Nanoarchaeal 16S rRNA gene sequences are widely dispersed in hyperthermophilic and mesophilic halophilic environments. Extremophiles. 12:651-656. [2.2/5]
  • Gutiérrez, MC, Castillo, AM, Pagaling, E, Heaphy, S, Kamekura, M, Xue, Y, Ma, Y, Cowan, DA, Jones, BE, Grant, WD, Ventosa, A. (2008) Halorubrum kocurii sp. nov., an archaeon isolated from a saline lake. Int. J. Syst. Evol. Microbiol. 58:2031-2035. [2.1/0]
  • Carrasco, IJ, M. C. Márquez, Y. Xue, Y. Ma, D. A. Cowan, B. E. Jones, W. D. Grant and A. Ventosa. (2008) Sediminibacillus halophilus gen. nov., sp. nov., a moderately halophilic Gram-positive bacterium from a hypersaline lake. Int. J. System. Evol. Microbiol. 58:1961-1967. [2.1/3]
  • Márquez, C, Carrasco, IJ, Xue, Y, Ma, Y, Cowan, DA, Jones, BE, Grant, WD and Ventosa, A. (2008) Aquisalibacillus elongatus gen. nov., sp. nov., a moderately halophilic bacterium of the family Bacillaceae isolated from a saline lake. Int. J. System. Evol. Microbiol. 58:1922-1926. [2.1/4]
  • Dürr, R., Neumann, A., Vielhauer, O., Altenbuchner, J., Burton, S.G., Cowan, D.A., Syldatk, C. (2008) Genes responsible for hydantoin degradation of a halophilic Ochrobactrum sp. G21 and Delftia sp. I24 - New insight into relation of d-hydantoinases and dihydropyrimidinases. J. Molec. Cataly. B: Enzymatic 52-53, pp. 2. [2.3/2]
  • Hu, L, Pan, H, Xue, Y, Ventosa, A, Cowan, DA, Jones, BE, Grant, WD, Ma, Y. (2008) Halorubrum luteum sp. nov., isolated from Lake Chagannur, Inner Mongolia, China. Int. J. System. Evol. Microbiol. 58:1705-1708. [2.1/4]
  • Taylor, M, Tuffin, M, Burton, SG, Eley, K, Cowan, DA. (2008) Microbial responses to solvent and alcohol stress. Biotechnol. J. 3:1388-1397 [(2.1)/5]
  • Cowan, DA. (2008) Biotechnology in South Africa. Biotechnol. J. 3:1322-1323 [(2.1)/0]
  • Babalola, OO, Kirby, BM, Le Roes-Hill, M, Cook, AE, Cary, SC, Burton, SG, Cowan, DA. (2009) Phylogenetic analysis of actinobacterial populations associated with Antarctic Dry Valley mineral soils. Envir. Microbiol. 11:566-576 [5.5/19]
  • le Roes-Hill, M, Rohland, J, Meyers, PR, Cowan, DA, Burton, SG. (2009) Streptomyces hypolithicus sp. nov., a novel streptomycete isolated from a hypolith community, Antarctica. Int. J. System. Evol. Microbiol. 59:2033-2036 [1.9/0]
  • Taylor, M, Eley, K, Martin, S, Tuffin, IM, Burton, SG, Cowan DA (2009) Thermophilic ethanologenesis. Future prospects for second generation ethanol production. Trends Biotechnol. 27:398-405 [9.6/27]
  • Ruiz Sebastián, C, Sink, KJ, McClanahan, TR, Cowan, DA (2009) Bleaching response of corals and their Symbiodinium communities in southern Africa. Marine Biology 156:2049-2062 [2.0/3]
  • Heath, C, Hu, XP, Cary, SC, Cowan, DA. (2009) Isolation and characterisation of a novel, low-temperature-active alkaliphilic esterase from an Antarctic desert soil metagenome. Appl. Environ. Microbiol. 75:4657-4659 [3.8/21]
  • Pagaling, E, Wang, H, Venables, M, Wallace, A, Grant, WD, Cowan, DA, Jones, BE, Ma, Y, Ventosa, A, Heaphy, S. (2009) Microbial biogeography of six salt lakes in Inner Mongolia and one salt lake in Argentina. Appl. Environ. Microbiol. 75:5750-5760 [3.8/5]
  • Tuffin, IM, Anderson, D, Heath, C, Cowan, DA (2009) Metagenomic gene discovery: How far have we moved in sequence space? Biotechnol. J. 4:1671-1683. [(2.1)/7]
  • Cowan, DA (2009) Cryptic microbial communities in Antarctic deserts. Proc. Natl. Acad. Sci, USA. 106:19749-19750 [9.8/5]
  • Cowan, DA, Tuffin, IM (2009) Capturing global metabolism. Nature Biotechnol. 27:1132-1133 [31.1/0]
  • Wong FK, Lau MC, Lacap DC, Aitchison JC, Cowan DA, Pointing SB (2010) Endolithic microbial colonization of limestone in a high-altitude arid environment. Microb. Ecol. 59:689-699 [2.9/2]
  • Cary, SC, McDonald, I, Barrett, JE and Cowan, DA. (2010) On the rocks: Microbial ecology of Antarctic cold desert soils. Nature Rev. Microbiol. 8:129-138. [20.7/36]
  • Wong, FKY, Lacap, DC, Lau, MCY, Aitchison, JC, Cowan, DA, Pointing, SB. (2010) Hypolithic microbial community of quartz pavement in the high-altitude tundra of central Tibet. Microb. Ecol. 60:730-739 [2.9/6]
  • Musingarimi, W., Tuffin, M., Cowan, D. (2010) Characterisation of the Arsenic Resistance Genes in Bacillus sp. UWC Isolated from Maturing Fly Ash-Acid Mine Drainage Neutralized Solids. S.A. J. Sci. 106:59-63. [0.6/0]
  • Staniland, S., Coppock, M., Tuffin, M., van Zyl, L., Roychoudhury, A. N., Cowan, D. (2010) Cobalt uptake and resistance to trace metals in Comamonas testosteroni isolated from a heavy-metal contaminated site in the Zambian Copperbelt. Geomicrobiol. J. 27:656-662 [1.7/x]
  • Bauer, R, Cowan, DA, Crouch, A. (2010) Acrolein in wine: Importance of 3-hydroxypropionaldehyde and derivatives in production and detection. J. Agric. Food Chem. 58:3243-3250. [2.8/0]
  • Barnard, D, Casanueva, A, Tuffin, IM, Cowan DA. (2010) Extremophiles in biofuels production. Environ. Technol. 31:871-888. [1.0/3]
  • Cowan, DA, Khan, N, Pointing, S, Cary, SC. (2010) Diverse hypolithic refuge communities in Antarctic Dry Valleys, Antarct. Sci. 22:714-720 [1.3/5]
  • Casanueva, A., Tuffin, IM, Cary, SC and Cowan, DA. (2010) Molecular adaptations to psychrophily: The impact of 'omic' technologies. Trends Microbiol. 18:374-381. [7.5/4]
  • Williamson, DS, Dent, KC, Weber, BW, Varsani, A, Frederick, J, Thuku, RN, Cameron, RA, van Heerden, JH, Cowan, DA, Sewell, BT. (2010) Structural and biochemical characterization of a nitrilase from the thermophilic bacterium, Geobacillus pallidus RAPc8. Appl. Microbiol. Biotechnol. 88:143-153. [3.6/0]
  • Velankar H, Clarke KG, du Preez R, Cowan DA, Burton SG. (2010) Developments in nitrile and amide biotransformation processes. Trends Biotechnol. 28:561-569 [9.6/1]
  • Meiring, T, Mulako, I, Tuffin, MI, Meyer, Q, Cowan, DA. (2010) Retrieval of full-length functional genes using subtractive hybridization magnetic bead capture. Methods Mol. Biol. 668:287-297. [13.9/0]
  • Olaofe, OA, Burton, SG, Cowan, DA, Harrison, STL (2010) Improving the production of a thermostable amidase through optimising IPTG induction in a highly dense culture of recombinant Escherichia coli. Biochem. Engineer. J. 52:19-24 [2.7/1]
  • Chiyanzu, I, Cowan, DA, Burton, SG (2010) Immobilization of a nitrile hydratase (NHase) from Geobacillus pallidus RAPc8 overcomes substrate inhibition and enhances stability. J. Mol. Catal. B, Enzymatic. 63:109-115. [2.3/3]
  • Cowan DA, Pointing SB, Stevens MI, Cary SC, Stomeo F, Tuffin IM. (2011) Distribution and abiotic influences on hypolithic microbial communities in an Antarctic Dry Valley. Polar Biol. 34:307–311. [1.4/4]
  • Taylor, MP, van Zyl, L, Tuffin, IM, Leak, DJ, Cowan, DA. (2011) Genetic tool development underpins recent advances in thermophilic whole-cell biocatalysts. Microb. Biotechnol. 4:438-448. [(2.1)/1]
  • Welz, PJ, Ramond, J-B, Cowan, DA, Prins, A, Burton SG. (2011) Ethanol degradation and the benefits of incremental priming in pilot-scale constructed wetlands. Ecol. Engineer. 37:1453-1459. [2.2/0]
  • Nel, AJM, Tuffin, IM, Sewell, BT, Cowan, DA. (2011) A unique aliphatic amidase from a psychrotrophic and haloalkaliphilic Nesterenkonia isolate. Appl. Environ. Microbiol. 77:3696-3702. [3.8/1]
  • Rohr, LM, Mashaphu, N, Sheridan, C, Burton, SG, and Cowan, DA. (2011) Seasonal shifts of the microbial community structure in a winery waste-impacted wetland soil. Trans. Roy. Soc. S.A. 66:41-53.
  • Cowan, DA, Sohm, JA, Makhalanyane, T, Capone, DG, Green, TGA and Cary, SC. (2011) Hypolithic communities: important nitrogen sources in Antarctic desert soils, Envir. Microbiol. Rep. 3:581-586 [3.1/0]
  • Khan, N, Tuffin, IM, Stafford, W, Cary, SC, Lacap, DC, Pointing, SB, Cowan, DA (2011) Hypolithic microbial community colonization of quartz rocks from Miers Valley, McMurdo Dry Valleys, Antarctica. Polar Biol. 34:1657-1668 [1.4/1]
  • Cowan, DA, Fernendez-Lafuente, R (2011) Enhancement of thermophilic enzymes by chemical modification and immobilization. A review. Enz. Microb. Technol. 49: 326-346 [2.3/0]
  • Cowan, DA, Chown, SL, Convey, P, Tuffin, IM, Hughes, K, Pointing, SB, Vincent, WF. (2011) Non-indigenous microorganisms in the Antarctic – assessing the risks. Trends Microbiol. 19:540-548 [7.5/0]
  • Ramond J-B, Welz P.J., Cowan D.A., Burton S.G. (2012) Microbial community structure stability, a key parameter in monitoring the development of constructed wetland mesocosms during start-up. Res. Microbiol. 163:28-35. [2.4/0]
  • Hu, XP, Heath, C, Taylor, MP, Tuffin, IM, Cowan, DA (2012). A novel, extremely alkaliphilic and cold active esterase from Antarctic desert soil. Extremophiles. 16:79-86 [2.2/0]
  • Tiao, G, Lee, CK, McDonald, IR, Cowan, DA, Cary, SC. (2012) Rapid microbial response to removal of the presence of an ancient relic in the Antarctic Dry Valleys. Nature Comms. 3: 360
  • Taylor, MP, Mulako, I, Tuffin, IM, Cowan, DA (2012).Understanding physiological responses to pre-treatment inhibitors in ethanologenic fermentations. Biotechnol. J. In Press [(2.1)]
  • Sanyika, WT, Stafford, W, Tuffin, IM, Cowan, DA. (2012) The soil and plant determinants of community structures of the dominant actinobacteria in Marion Island terrestrial habitats, Sub-Antarctica. Polar Biol. DOI 10.1007/s00300-012-1160-0 [1.4/0]
  • Szeker, K, Zhou, X, Schwab, T, Casanueva, A, Cowan, D, Mikhailopulo, IA, Neubauer, P. (2012) Comparative investigations on thermostable pyrimidine nucleoside phosphorylases from Geobacillus thermoglucosidasius and Thermus thermophilus. Journal of Molecular Catalysis B: Enzymatic. In Press
  • Stomeo, F, Makhalanyane, T, Valverde, A, Pointing, S, Stevens, M, Cary, SC, Tuffin, Cowan, DA (2012) Abiotic factors influence microbial diversity in permanently cold soil horizons of a maritime-associated Antarctic Dry Valley. FEMS Microb. Ecol. Mar 19. doi: 10.1111/j.1574-6941.2012.01360
  • Pagaling E, Grant WD, Cowan DA, Jones BE, Ma Y, Ventosa A, Heaphy, S. (2012) Bacterial and Archaeal diversity in two hot spring microbial mats from the geothermal region of Tengchong, China. Extremophiles, Accepted for publication
  • di Prisco, G, Convey, P, Gutt, J, Cowan, DA, Conlan, K, Verde, C. (2012) Understanding and Protecting the World's Biodiversity: the Role and Legacy of the SCAR Programme "Evolution and Biodiversity in the Antarctic”. Marine Genomics, In Press.
  • Chown,SL, Lee, JE, Hughes, KA, Barnes, J, Barrett, PJ, Bergstrom, DM, Convey, P, Cowan, DA, Crosbie, K, Dyer, G, Frenot,Y, Grant, SM, Herr, D, Kennicutt, MC, Lamers, M, Murray, A, Possingham, HP, Reid, K, Riddle, MJ, Ryan, PG, Sanson, L, Shaw, JD, Sparrow, MD, Summerhayes, C, Terauds, A, Wall, DH. (2012) Challenges to the Future Conservation of the Antarctic. Science, Accepted for publication.
  • Valverde, A, Tuffin, IM, Cowan, DA. (2012) Biogeography of bacterial communities in hot springs: a focus on the actinobacteria. Extremophiles, Accepted for publication
  • Welz, PJ, Ramond, J-B, Cowan, DA, Burton, SG (2012) Phenolic removal processes in biological sand filters, sand columns and microcosms. Biores. Technol., Accepted for publication.
  • Meiring, T, Tuffin, IM, Cowan, DA. (2012) Genome sequence of temperate
    bacteriophage Psymv2 from Antarctic Dry Valley soil isolate
    Psychrobacter sp. MV2" Thermophiles Accepted for publication.
  • Lacap, DC, Chan, Y, Lau, MCY, Warren-Rhodes, KA, Cockell, CC, Cowan, DA, McKay, CP, Pointing, SB. Hypolithic microbial communities: Between a rock and a hard place. ISME J., Accepted for publication.
  • Khazir, J, Mir, BA, Mir, SA, Cowan, D. (2014) Natural products as lead compounds in drug discovery. J. Asian Nat. Prod. Res. 15: 764-788
  • Ramond J-B, Welz P, Le Roes-Hill M, Tuffin IM, Burton SG, Cowan, DA. (2014)  Selection of Clostridium spp. in biological sand filters neutralizing synthetic acid mine drainage. FEMS Microb. Ecol.   87: 678-690
  • Welz PJ, Ramond J-B, le Roes-Hill M, Cowan DA, Burton SG (2014) Minor differences in sand physicochemistry lead to major differences in bacterial community structure and function after exposure to synthetic acid mine drainage. Biotechnol Bioproc Engineer. 19:211-220
  • Mir, BA, Mewalal, R, Mizrachi, E, Myburg, AA, Cowan, DA. (2014) Recombinant hyperthermophilic enzyme expression in plant tissues: a novel approach for lignocellulose digestion.  Trends Biotechnol.  32: 281-289
  • Guerrero LD, Makhalanyane TP, Aislabie JM, Cowan DA. (2014) Draft genome sequence of Williamsia sp. strain D3, isolated from the Darwin Mountains, Antarctica. Genome Announc. 2: e01230-13
  • De Maayer, P, Anderson, DE, Cary, SC, Cowan, DA, (2014) Some like it cold: understanding the strategies of psychrophiles: EMBO Rep. 15: 508-517
  • Aliyu H, De Maayer P, Rees DJG, Tuffin IM, Cowan DA. (2014) The draft genome sequence of Antarctic poly-extremophile Nesterenkonia sp. AN1. Genome Announc. 2:e00197-14
  • Mir BA, Khazir J, Hakeem KR, Koul S, Cowan DA (2014) Enhanced production of withaferin-A in shoot cultures of Withania somnifera (L) Dunal.  J. Plant Biochem. Biotechnol.  23: 430-434
  • Biely P, Westereng B, Puchart V, de Maayer P, Cowan DA (2014) Recent progress in understanding the mode of action of acetylxylan esterases. J. Appl. Glycosci. 61: 35-44.
  • Adriaenssens EM, Guerrero LD, Makhalanyane TP, Aislabie JM, Cowan DA. (2014) Draft genome sequence of the aromatic hydrocarbon-degrading bacterium Sphingobium sp. strain Ant17 isolated from Antarctic soil. Genome Announc. 2:e00212-14
  • Adriaenssens EM, Cowan DA (2014) Using signature genes as tools to assess environmental viral diversity and ecology.  Appl. Environ. Microbiol.  80:4470-4480
  • Cowan DA, Makhalanyane TP, Dennis PG, Hopkins DW. (2014) Microbial ecology and biogeochemistry of continental Antarctic soil. Frontiers Syst. Microbiol. 5:154
  • De Maayer, P, Williamson, C, Vennard, C, Danson, M, Cowan, D. (2014) The draft genomes of Geobacillus spp. CAMR5420 and CAMR12739. Genom. Announc.  2:e00567-14
  • Gunnigle, E,  Ramond, J-B, Frossard, A, Seeley, M, Cowan, DA. (2014) Sequential co-extraction method for DNA, RNA and protein recovery from soil for future system-based approaches.  J. Microbiol. Method. 103:118-123.
  • Kennicutt, MC, Chown SL, Cassano, J, Liggett, D, Massom, R, Peck, L, Rintoul, S, Storey, J, Vaughn, D, Wilson, T, Sutherland WJ, Allison, I, Ayton, J, Badhe, R, Baeseman, J, Barrett, P, Bell, R, Berthler, N, Bo, S, Brandt, A, Bromwich, D, Cary C, Cassano, J, Clark, M, Convey, P, Costa, E, Cowan, DA, DeConto, R, Dunbar, R, Elfring, C, Escutia, C, Francis, J, Fricker, H, Fukuchi, M, Gilbert, N, Gutt, J, Havermans, C, Hik, D, Hosie, G, Jones, C, Kim, Y, Le Maho, Y, Lee, S-H, Leppe, M, Leychenko, G, Li, X, Liggett, D, Lipenkov, V, Lochte, K, Lopez-Martinez, J, Ludecke, C, Lyons, B, Maranessi, S, Miller, H, Morozova, P, Naish, T, Nayak, S, Ravindra, R, Retamales, J, Ricci, C, Rogon-Finnimore, N, Ropert-Coudert, Y, Samah, A, Sanson, L, Scambos, T, Schloss, I, Shiraisha, K, Siegert, M, Simoes, J, Sparrow, M, Storey, B, Wall, D, Walsh, J, Wilson, G, Winther, J-G, Xavier, J, Yang, H. (2014) Six priorities for Antarctic science. Nature,  512, 23–25.
  • De Maayer, P, Brumm, PJ, Mead DA, Cowan, DA. (2014) Comparative analysis of the Geobacillus hemicellulose utilization locus reveals a highly variable target for improved hemicellulolysis. BMC Genomics. 15:836
  • Ferreras, E, De Maayer, P, Makhalanyane, TP, Guerrero, L, Aislabie, J, Cowan, DA. (2014) The draft genome sequence of Microbacterium sp. CH12i, isolated from shallow groundwater in Cape Hallet, Antarctica. Genom. Announc. 2:e00789-14
  • van Zyl, L, Schubert, W-D, Tuffin, IM, Cowan, DA. (2014) Functional and structural characterization of a pyruvate decarboxylase from Gluconoacetobacter diazotrophicus. BMC Struct. Biol. 14:21
  • Kennicutt, MC, SL Chown, J Cassano, D Liggett, LS Peck, R Massom, SR Rintoul, J Storey, DG Vaughan, TJ Wilson, I Allison, J Ayton, R Badhe, J Baeseman, PJ Barrett, RE Bell, N Bertler, S Bo, A Brandt, D Bromwich, C Cary, M S Clark, P Convey, ES Costa, D Cowan, R Deconto, R Dunbar, C Elfring, C Escutia, J Francis, H A Fricker, M Fukuchi, N Gilbert, J Gutt, C Havermans, D Hik, G Hosie, C Jones, YD Kim, Y Le Maho, Sh Lee, M Leppe, G Leitchenkov, X Li, V Lipenkov, K Lochte, J López-Martínez, C Lüdecke, W Lyons, S Marenssi, H Miller, P Morozova, T Naish, S Nayak, R Ravindra, J Retamales, CA Ricci, M  Rogan-Finnemore, Y Ropert-Coudert, AA Samah, L Sanson, T Scambos, IR Schloss, K Shiraishi, MJ Siegert, J Simões, MD Sparrow, B Storey, DH Wall, JC Walsh, G Wilson, JG Winther, J C Xavier, H Yang, WJ Sutherland (2014) A roadmap for Antarctic and Southern Ocean science for the next two decades and beyond. Antarct. Sci. 27: 3-18.
  • Zablocki O, van Zyl L, Adriaenssens EM, Tuffin IM, Cary SC, Cowan DA. (2014) High-level diversity of tailed phages, eukaryotic-associated viruses and virophage-like elements in the metaviromes of Antarctic soils. Appl. Environ. Microbiol. 80: 6888-6897.
  • Zablocki, O, Rybicki, E, Cowan, DA. (2014) First report of a potyvirus infecting Albuca rautanenii in the Namib Desert. Plant Disease. 98: 1749
  • Ramond J-B, Pienaar A, Armstrong A, Seely M, Cowan DA (2014) Niche-partitioning of edaphic microbial communities in the Namib Desert gravel plain Fairy Circles. PLoS One, 9: 1-6.
  • Weller-Stuart, A, Chan, WY, Coutinho, TA, Venter, SN, Smits, THM, Duffy, B, Cowan, DA, de Maayer, P (2014) Draft genome sequences of the onion centre rot pathogen P. ananatis PA4 and maize brown stalk rot pathogen P. ananatis BD442. Genome Announcement. 2: 1-2.
  • de los Ríos, A., Cary, C., Cowan, DA. (2014) The spatial structures of hypolithic communities in the Dry Valleys of East Antarctica. Polar Biol. 37: 1823-1833.
  • Zablocki O, van Zyl L, Adriaenssens EM, Rubagotti E, Tuffin IM, Cary SC, Cowan DA. (2014) Niche-dependent genetic diversity in Antarctic metaviromes. Bacteriophage 4, e980125
  • Valverde A, Makhalanyane TP, Cowan DA (2014) Contrasting assembly processes in a bacterial metacommunity along a desiccation gradient. Front. Microbiol. 5: 668.
  • Adriaenssens, E, van Zyl, L, De Maayer, P, Rubagotti, E, Rybicki, E, Tuffin, IM, Cowan, DA (2015) Metagenomic analysis of the viral community in Namib Desert hypoliths.  Environ. Microbiol. 17:480-495.
  • Ramond, J-B, Makhalanyane, TP, Tuffin, IM, Cowan, DA. (2015) Normalization of environmental metagenomic DNA enhances the discovery of novel microbial community members. Lett. Appl. Microbiol. 60: 359-366
  • Valverde, A, Makhalanyane, TP, Seely, M, Cowan, DA.  (2015) Cyanobacteria drive food-webs and functionality in niche soil communities. Molec. Ecol.  24: 812-821
  • Makhalanyane T, Valverde A, Gunnigle E, Frossard A, Ramond J-B, Cowan DA   (2015) Microbial ecology of hot desert edaphic systems.  FEMS Microbiol. Rev.  39: 203-221
  • Ramond JB, Lako JDW, Stafford WHL, Tuffin IM, Cowan DA  (2015) Evidence of novel plant-species specific ammonia oxidizing bacterial clades in acidic South African fynbos soils.  J. Basic Microbiol.  54: 1-8
  • Wei STS, Higgins CM, Adriaenssens EM,  Cowan DA, Pointing SB (2015) Genetic signatures indicate widespread antibiotic resistance and phage infection in microbial communities of the McMurdo Dry Valleys, East Antarctica. Polar Biol. 38: 919-925
  • Gombeer S, Ramond J-B, Eckardt F, Seely M, Cowan DA   (2015) The influence of soil physicochemistry on the edaphic bacterial communities in contrasting terrain types of the Central Namib Desert. Geobiology  13:494-505
  • Gunnigle E, Ramond J-B, Guerrero LD, Makhalanyane TP, Cowan DA (2015) Draft genomic DNA sequence of the multi-resistant Sphingomonas sp. strain AntH11 isolated from an Antarctic hypolith. FEMS Microbiology Letters 362 (8): fnv 037
  • Hughes KA, Cowan DA, Wilmotte A (2015) Protection of Antarctic microbial communities – ‘out of sight, out of mind’. Frontiers Microbiol.  6: 151
  • Quiroga MV, Valverde A, Mataloni G, Cowan D. (2015) Understanding diversity patterns in bacterioplankton communities from a Sub-Antarctic peatland. Environ. Microbiol. Rep. 7: 547-553
  • Makhalanyane TP, Valverde A, Velazquez D, Gunnigle E, Van Goethem M, Quesada A, Cowan D. (2015) Ecology and biogeochemistry of cyanobacteria in soils, permafrost, aquatic and cryptic polar habitats. Biodiv. Conservat.  24: 819-840
  • Brumm P, De Maayer P, Cowan DA, Mead DA (2015) Genomic analysis of six new Geobacillus strains reveals highly conserved carbohydrate degradation architectures and strategies. Frontiers Microbiol.  6: 430
  • De Maayer P, Chan WY, Martin DAJ,   Blom J, Venter SN,   Duffy B,   Cowan DA, Smits THM, Coutinho TA (2015) The Pantoea ananatis integrative conjugative element ICEPan plays a potential role in its ecological diversification and antibiosis. Frontiers Microbiol. 6:576
  • Van Zyl L, Cowan DA, Trindade IM (2015) Identification and characterization of a novel Geobacillus thermoglucosidasius bacteriophage, GVE3. Arch. Virol. 160: 2269-2282
  • Cowan DA, Ramond J-B, Makhalanyane TP, De Maayer P. (2015) Metagenomics of extreme environments. Current Opin. Microbiol. 25: 97–102
  • Ohlhoff, CW, Kirby, BM, Casanueva, A, Huddy, RJ, Bauer, R, Mutepfa, DLR, Cowan, DA, Tuffin, IM. (2015) An unusual feruloyl esterase belonging to family VIII esterases and displaying a broad substrate range.  J. Mol. Cataly. B.  118: 79-88
  • Ronca S, Ramond J-B, Cowan, DA   (2015) Microbial community structure along dune-interdune transects in the Namib Desert. Frontiers Microbiol.  6: 845
  • Frossard A, Ramond J-B, Seely M, Cowan DA. (2015) Water regime history drives responses of soil Namib Desert microbial communities to wetting events. Scientific Reports  5: 571-578
  • Anderson DE, Ferreras E, Tuffin IM, Cowan  DA  (2015)  A novel bacterial Water Hypersensitivity response protein shows in vivo protection against cold and freeze damage. FEMS Microbiol. Lett. 362: fnv110
  • Mir BA, Mir SAKhazir JTonfack LBCowan DAVyas DKoul S (2015) Cold stress affects antioxidative response and accumulation of medicinally important withanolides in Withania somnifera (L.) Dunal. Indust. Crops Products 74: 1008-1016
  • Bezuidt O, Makhalanyane, TP, Gomri MA, Kharroub K, Cowan DA (2015) Draft genome sequence of thermophilic Geobacillus sp. strain Sah69, isolated from Saharan soil, southeast Algeria. Genome Announc. 3: e01447-15
  • Vikram S, Guerrero L, Makhalanyane TP, Le P-T, Seely M, Cowan DA. (2016) Metagenomic analysis provides insights into functional capacity in a hyperarid desert soil niche community.  Environ. Microbiol.  18: 1875 - 1888
  • Zablocki O, Adriaenssens E, Cowan DA. (2016) Diversity and ecology of viruses in hyperarid desert soils.  Appl. Environ. Microbiol. 82: 770-777
  • Adriaenssens, EM, Van_Zyl, LJ, Cowan, DA, Trindade M. (2016) Namib Desert salt pans: a novel lineage of haloarchaeal salterproviruses  and a rich source of ssDNA viruses. Viruses.  8 (1): DOI: 10.3390/v8010014
  • Makhalanyane TP, Van Goethem M, Cowan DA. (2016) Microbial diversity and functional capacity in polar soils. Curr Opin Biotechnol.  38: 159 -166
  • Aliyu H, De Maayer P, Cowan DA  (2016) The genome of the Antarctic polyextremophile Nesterenkonia AN1 reveals adaptive strategies for survival under multiple stress conditions. FEMS Microb Ecol. 92: fiw032
  • Van Goethem M, Makhalanyane T, Valverde A, Cary S, Cowan D.  (2016) Characterization of bacterial communities in lithobionts and soil niches from Victoria Valley, Antarctica.  FEMS Microb Ecol. In Press
  • Wilcox D, Cowan DA (2016) Diversity of Frankia in root nodules of six Morella from the Cape flora of South Africa. Plant Soil  In Press
  • Valverde A, De Maayer P, Oberholster T, Henschel J, Louw MK,  Cowan DA. (2016) Specific microbial communities associate with the roots of Welwitschia mirabilis, a living fossil. PLoS One, 11: e0153353.
  • Beet C, Hogg I, Collins G, Cowan D, Wall D, Adams B. (2016) Genetic diversity among populations of Antarctic springtails (Collembola) within the Mackay Glacier ecotone. Genome. 10.1139/gen-2015-0194
  • De Maayer P, Cowan DA (2016) Flashy flagella: flagellin modification is relatively common and high versatile among the Enterobacteriaceae.  BMC Genomics.  17: 377
  • Oloo F, Valverde A, Quiroga MV, Vikram S, Cowan DA, Mataloni G. (2016) Habitat heterogeneity and connectivity shape aquatic microbial communities in South American peatlands. Scientific Reports. 6: 25712
  • van der Walt A, Johnson R, Cowan D, Seely M, Ramond J-B (2016) Unique microbial phylotypes in Namib Desert Fairy Circle soils. Appl Environ Microbiol. Accepted for publication
  • Bezuidt OKI, Pierneef R, Gomri AM, Adesioye F, Makhalanyane TP, Kharroub K, Cowan DA. (2016) The Geobacillus pan-genome: Implications for evolution of the genus. Frontiers Microbiol.  7: 723
  • Miyambo, T, Makhalanyane, T, Cowan, D, Valverde, A.  (2016) Plants of the Fynbos biome harbour host species-specific bacterial communities.  FEMS Micro. Lett.  Accepted for publication.
  • Adesioye FA, Makhalanyane TP, Beily P, Cowan DA (2016) Phylogeny, classification and metagenomic bioprospecting of microbial acetyl xylan esterases.  Enzyme Microb. Technol. Accepted for publication.
  • Franzini PZN, Ramond J-B, Scholtz CH, Sole CL, Ronca S, Cowan DA. (2016) The gut microbiomes of two Pachysoma MacLeay desert Dung Beetle species (Coleoptera: Scarabaeidae: Scarabaeinae) feeding on different diets. PLoS One, Accepted for publication.
  • Phuong Thi Le, Makhalanyane TP, Guerrero L, Vikram S, Van de Peer Y, Cowan DA. (2016) Comparative metagenomic analysis reveals mechanisms for stress response in hypoliths from extreme hyperarid deserts. Genome Biol. Evol. Accepted for publication
  • De Scally S, Frossard A, Hogg I, Cowan DA, Makhalanyane T. (2016) Antarctic microbial communities are functionally redundant, adapted and resistant to short term temperature perturbations. Soil Biol.  Biochem. Accepted for publication
Last edited by Kishen Mahesh

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